Identification and characterization of transcription factors regulating methionine-derived glucosinolate biosynthesis II

Abstract

We previously reported that PMG1 and PMG2 are transcription factors regulating methionine-derived glucosinolates (MET-GSL) biosynthesis (Hirai et al., 2007). In this study, we analyzed the expression of PMG1, PMG2 and a novel candidate transcription factor, PMG3, in knockout lines of PMG1 and PMG2. Expression of PMG2, and PMG3 was reduced to 30%, and 10%, respectively, in pmg1 in which MET-GSLs were decreased. In pmg1pmg2 in which MET-GSLs were not detected, expression of PMG3 was reduced to 5%. On the other hand, in pmg2 containing comparable amount of MET-GSLs to wild-type, the amount of transcripts of PMG1, and PMG3 was 100%, and 40%, respectively, of those in wild-type. These results suggest that PMG3 is regulated by PMG1 and PMG2 and involves in the regulation of MET-GSL biosynthesis, although PMG1 is a principal regulator.