Abstract
Studies of plant metabolic response against pathogens have focused on a small number of signaling metabolites. Few comprehensive analyses have been performed to obtain further insights into the metabolic regulation in response to pathogen. Here, we performed comprehensive metabolic profiling of Arabidopsis lines that have different levels of virus resistance.
By expressing a resistance gene RCY1 in Columbia, which is susceptible to Cucumber mosaic virus [CMV(Y)], we obtained ER (extreme resistance) line and HR (hypersensitive response) line. We found that ER line demonstrated no difference, whereas HR line demonstrated significant difference between metabolic profiles of mock- and CMV(Y)-inoculated plants at 24 hours after inoculation. Columbia showed the largest difference between mock- and CMV(Y)-inoculation. This indicates that the metabolic change associated with CMV(Y)-inoculation depends on the strength of resistance. In non-inoculated plants, ER line showed a metabolic profile distinct from other two lines, suggesting that ER line constitutively accumulates resistance-related metabolites.
