Metabolome analysis of Chara vacuole

Abstract

We have developed the metabolome analysis system for one vacuole of Chara corallina, including the methods of isolating one vacuole from one internodal cell of Chara corallina and the metabolome analysis using capillary electrophoresis / mass spectrometry (CE-MS). We isolated over 10 μL of the internal fluid of one vacuole from one Chara internodal cell by use of a glass capillary. The vacuolar fluid was directly introduced into CE-MS system without any sample preparation steps. We detected approximately 1,000 peaks from one vacuolar sample on the cation and anion mode of CE-MS analysis. Among them, some peaks were identified as known metabolites, such as amino acids or organic acids, by the verification using both m/z value and migration time of standard compounds. Moreover, we will report the metabolic disturbance in a Chara vacuole caused by various environmental stresses.