Salt Accumulation Mechanism Into The Vacuole In Plants Cells Under Salt Stress

Abstract

Most of higher plants can not grow under high salt condition. However, some plants (e.g. mangrove) can tolerate high salt condition. Salt accumulation in the vacuoles is one of the mechanisms for salt tolerance. During salt accumulation, a rapid increase of vacuolar volume without increasing cell-size was reported in barely roots and mangrove suspension cultured cells.
In this study, we analyzed behaviors of membrane vesicles and vacuoles in Arabidopsis under high salt condition. High salt condition induced a rapid increase in the vacuolar volume and activated dynamic state of the acid vesicles in Arabidopsis root cells.The same phenomenon of the vacuole was observed in Arabidopsis suspension-cultured cells. Using fluorescence Na⁺ indicator, we observed Na⁺ accumulation in the vacuole and dot-like fluorescence around the vacuole under high salt condition. We further analyze the dynamics of vesicles and the ion transporter proteins under high salt condition.