Host: The Japanese Society of Plant Physiologists
Pages 0253
Boron (B) cross-links pectic polysaccharides at rhamnogalacturonan II regions and thereby contributes to build the cell wall structure. However, it remains unknown how B deficiency causes various metabolic disorders and brings about cell death. To understand this mechanism, we have analyzed the response of cultured tobacco BY-2 cells to B deprivation.
When 3-day-old cells were transferred to a B-free medium, dead cells were evident as early as 12 h after the treatment. We previously showed that the genes for antioxidative enzymes are upregulated in low B-acclimated cells, which suggests that oxidative damages are involved in low B stress. Here we found that lipid peroxides and reactive oxygen species were accumulated in -B cells. In addition, an antioxidant butylated hydroxyanisole reduced cell death. These results indicate that oxidative damage does occur under B deficiency as the direct cause of cell death.