Host: The Japanese Society of Plant Physiologists
Pages 0254
To remediate the worldwide-spreading acid-sulphate soil area and utilize it for human beings, acid tolerant leguminous plants are expected to be useful as crops and pioneer plants. We report the isolation of acid-tolerant genes by functional cloning of a soybean cDNA library and their characterization in planta. E. coli cells were transformed with a cDNA library of soybean constructed with the pDEST14 expression vector and cultured on a LB medium adjusted to pH 4 or pH 5. A total of 27 unique resistant clones were isolated. Some of the corresponding genes in soybean seedlings were constitutively expressed, while others were induced by the aluminium stress. Selected six clones were introduced into A. thaliana using the pGWB2 vector. All the clones tested were able to confer acid tolerance to A. thaliana, that is, the overexpressors grew better than the wild type under the acid and aluminium stresses.
