Host: The Japanese Society of Plant Physiologists
Pages 0276
Yieldin (YLD) is the wall protein, isolated from cowpea cell wall (Okamoto-Nakazato et al. 2000), regulating the wall yield threshold (y). Since the molecular cloning study of YLD exhibited that a carbohydrate-binding site was conserved within the YLD molecule, we have assumed YLD to have a carbohydrate-binding activity. To confirm the assumption, we analyzed the carbohydrate-binding activity of YLD by a hemagglutination test using rabbit red blood cells.
As the results, YLD was shown to have a hemagglutinating activity (HA activity) towards the trypsinated rabbit red blood cells and suggested to bind to the carbohydrate(s) on the surface of the red blood cells. The pH-dependency of the HA activity of YLD was similar to that of the YLD-regulated y in the cell wall. These results suggest that YLD is a kind of lectin and the carbohydrate-binding activity of YLD is related to the y-regulation in the cell walls.
