Host: The Japanese Society of Plant Physiologists
Pages 0275
Cell walls of higher plants are actively metabolized during cell growth. We had reported that the degradation of galactan occurred during cell elongation growth by using cell suspension cultures of poplar cells. In this study, I purified a galactan degrading enzyme from the medium of cultured Populus cells and some properties of the enzyme were revealed. The galactan degrading activity in the medium increased rapidly in late stage of elongation phase (10-14 days after subculture). The enzyme protein was purified from the medium on the 14-day to about 100-fold by anion-exchange, hydrophobic, cation-exchange, and gel permeation chromatography. The purified enzyme showed about major 70 and minor 66 kDa bands on SDS-PAGE. The molecular weight of major one agreed with one of the galactan degrading enzymes purified from cell walls. The optimum temperature and pH were 40˚C and 5.5 respectively. Other some properties will be reported in the meeting.
