Host: The Japanese Society of Plant Physiologists
Pages 0284
Our recent research suggested that LSD1 has a function to regulate indirectly the transcriptional control of genes involved in PCD by interacting with other proteins. To make the function of LSD1 clear more, we have focused on IAA8, which was previously identified as LSD1-interactor by yeast two-hybrid (Y2H) screening. Y2H analysis demonstrated that IAA8, one of Aux/IAA family proteins, interacts with LSD1 via same domain as with TIR1. Similarly, all Aux/IAAs with this domain we examined interact with LSD1, too. On the other hand, all LSD1-interactors should be localized in cytoplasm because LSD1 is localized exclusively in cytoplasm of plant cells. However, since only IAA8 is localized in cytoplasm of Arabidopsis mesophyll protoplasts, we suggest that LSD1 interacts only with IAA8 in vivo. By mutant analysis, IAA8 negatively regulated PCD induced in lsd1 mutant. This result strongly suggests that the function of IAA8 would participate in plant PCD inducing mechanism
