Host: The Japanese Society of Plant Physiologists
Pages 0285
The function of Arabidopsis bZIP transcription factor AtbZIP10 involved in programmed cell death is regulated by inhibition of its nuclear transport due to direct interaction with cytoplasmic retention protein LSD1. We also demonstrated that AtbZIP10 interacts with microtubule-plus-end-binding protein AtEB1. In this study, we tried to clarify the overview of functional control mechanism of AtbZIP10 by focusing on intracellular trafficking and selective degradation, which are important mechanism for precise transcriptional regulation. Using AtEB1 KO-mutant (ateb1a/b/c), we found that the function of AtEB1s does not participate in cytoplasmic location of AtbZIP10. In addition, because the phenotype of yeast bZIP transcription factor YAP1 mutant was partially complemented by AtbZIP10, the nucleocytoplasmic transport of AtbZIP10 may be regulated by intracellular redox state as YAP1. Furthermore, we supposed the presence of degradation mechanism for AtbZIP10 in nucleus and identified domain involved in degradation on AtbZIP10, which were examined based on the fluorescence of AtbZIP10-GFP.
