Analysis of enlarged fil-expression domain1 (enf1) Mutant Defective in Establishment of the Adaxial-Abaxial Axis in Leaves

Abstract

In Arabidopsis, leaves have the adaxial (upper side) and abaxial sides (lower side), which differ in shapes of cells and in the number of stomata or trichomes. In order to examine genetic mechanism determining the adaxial/abaxial axis in leaf primordia, we have isolated a series of mutants which show abnormal expression pattern of the abaxial specific gene, FILAMENTOUS FLOWER (FIL). enlarged fil-expression domain1 (enf1), has two types of leaves with abnormal FIL expression pattern: the one with enlarged FIL-expression domain and the other with reduced FIL-expression domain. Map-based cloning reveals that ENF1 gene encodes a metabolic enzyme. We will also report the expression pattern of ENF1 gene.