Abstract
Loss-of-function mutations in the ASYMMETRIC LEAVES2 (AS2) gene in Arabidopsis result in various defects in leaf development, such as formation of asymmetrically lobed and downwardly curled leaves, formation of leaflet-like structures from petioles, generation of an abnormal venation system, shorter length of petioles and leaf laminas than WT, and ectopic expression of class1 knox genes in the leaves. To uncover the function of AS2 in the leaf development, we screened enhancer and suppressor mutants of as2. We obtained several enhancer mutants and no suppressor mutant of as2, suggesting several pathways downstream of AS2 in leaf development. The enhancers of as2 could be divided into three types; formation of filamentous and trumpet-like leaves, increased formation of asymmetrically lobed and leaves, and shorter length of petioles and leaf laminas than as2. We will report the phenotype of these enhancer mutants of as2.
