Abstract
The TGN is one of the most important organelles for protein transport at the post-Golgi network, and functions as a sorting spot that directs cargo proteins to a variety of post-Golgi compartments. However, the TGN of plant cells has not been well understood yet. In order to elucidate the structure, function and dynamics of plant TGN, we focused on SYP4 group (SYP41, SYP42, SYP43), the ortholog of Tlg2/syntaxin16 which is localized to the TGN in yeast and mammalian cells, as a TGN marker. First, we established the transgenic plants expressing GFP-SYP4s under the control of the native promoter. The observation by CLSM showed that the TGN is a dot-like BFA (brefeldin A)-sensitive and Wm (wortmannin)-insensitive compartment, which is partially stained with FM4-64. These results suggest that the TGN functions in endocytic pathways in addition to the biosynthetic pathways. We will also report the phenotype of each mutant (syp41, syp42, syp43).
