Abstract
Plant hormones play an important role as signaling molecules in the regulation of almost all phases of plant development. They regulate expression of genes via each signal transduction systems, and in most cases they mutually regulate the signaling and metabolic systems between the hormones. Such complicated regulatory systems enable plant to grow and develop normally. To understand the fine regulatory systems at molecular and hormonal levels, we have established a method for high-throughput analysis of major plant hormones namely, cytokinins, auxins, abscisic acid, and gibberellins. Our method needs 10 to 100 mg fresh weight of plant tissues for determination and enables us to analyze 180 samples at a time. Using our method, we analyzed FOX hunting Arabidopsis lines overexpressing rice full-length cDNA and a variety of rice and Arabidopsis mutants. We will present the analyzed data and discuss the correlation between the morphological phenotypes of the mutants and hormone profiles.
