Abstract
Oxylipin, 9-hydroxy-10-oxo-12(Z), 15(Z)-octadecadienoic acid (KODA) is one of signal compounds which was mediated from the linolenic acid. It was found that KODA has a function to give stress tolerance and to activate inflorescence development in some species. In KODA's biosynthesis pathway, it has been suggested that the precursor dehydrated by two kinds of enzymes (LOX, AOS) like Jasmonate (JA). And it was also suggested that there were some differences in enzyme activity of biosynthesis between KODA and JA. Namely 9-specific oxidation enzymes were necessary in KODA biosynthesis instead of 13-specific enzyme. According to the current study, 9-specific enzyme identified that tissue-specific occurrence in vascular bundle in Lycopersicon esculentum and Solanum tuberosum. However there was little information of 9-specific enzyme in Arabidopsis thaliana. To detect 9-specific enzyme activity in Arabidopsis, We have done the functional analysis using by KODA.