Abstract
To reveal functions of a large population of rice genes efficiently, we are taking advantage of Full-length cDNA Over-eXpresser gene (FOX) hunting system. During generation of FOX-rice lines, we obtained two green calli on a 2,4-D-containing medium, on which wild-type rice calli are ivory yellow. OsGLK1 gene, which encodes a GARP-transcription factor, was ectopically overexpressed in the two calli in which chloroplasts with well-developed grana stacks were differentiated [Nakamura et al. (2007) PCP 48(Suppl): s117]. OsGLK1-FOX calli produced oxygen and were able to grow on sucrose-depleted media. These results suggested that developed chloroplasts in the OsGLK1 rice calli are functionally active, and that OsGLK1 is a key regulator of chloroplast development. To dissect OsGLK1-regulated genes, we performed microarray analysis of the OsGLK1-FOX line using the rice 44k oligo microarray. Based on the detailed analysis of microarray data, we will discuss the role of OsGLK1 in chloroplast development.
