Abstract
We have previously isolated a high-light and heat-shock-inducible gene, heat shock transcription factor (HsfA2). HsfA2 induces expressions of various defense-related genes, including HSPs, APX2, and GolS1 under environmental stress conditions, suggesting that HsfA2 is a key regulator in the induction of the defense system under oxidative conditions (Plant J., 2006). Here we investigated the gene regulation system via the HsfA2. The transient reporter assay using luciferase reporter constructs with different fragments of HSP18.1-CI, GolS1 and BAG promoter showed that the deletion of the sequence from -137, -290, and -92 bp to TATA box, respectively, markedly reduced the luciferase activity. Consequently, it seems likely that the presence of multiple HSEs in the proximal portion of the TATA box is essential for a high-level transcriptional activation by HsfA2.
