Abstract
CRES-T is a dominant and efficient gene silencing system which represses the target gene expression even in the presence of functionally-redundant transcription factors. Our aim is to provide informations for the application of this system to other plant species, as well as to provide useful materials for molecular biology and breeding through the concentrated study on flowers. Though we have succeeded in conferring the useful traits to Chrisanthemum and Torenia in many cases (e.g., using the chimeric repressors of TCP3, EIN3 and AP1) over half of the rest ended in unexpected phenotypes or no phenotype. This means the requirement of optimization of the gene constructs and/or the screening procedure. Here we show the availability of a novel screening method called the Bulk-Screening, in addition to our latest data on the CRES-T-applied transgenic flowers. This study is supported by the Research Project for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries.
