Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 49
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Host: The Japanese Society of Plant Physiologists

Quantification of 2'-deoxymigineric acid and Nicotianamine in rice xylem sap using LC/ESI-TOF-MS
*Yusuke Kakei, Yasuaki Wada, Isomaro Yamaguchi, Michiko Takahashi, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa
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Abstract
Nicotianamine (NA) chelates many kinds of metals and has a significant role in metal homeostasis in higher plants. 2'-deoxymugineric acid (DMA) is a chelater of Fe3+ and plays an important role in uptake of Fe in gramineous plants. According to previous works, citrate and histidine are suggested as ligands for metal transportation via xylem in Arabidopsis. In gramineous plants, DMA is also observed in xylem sap; hence DMA is supposed to play a role in metal transportation in xylem. We additionally determined NA in rice xylem sap using LC/ESI-TOF-MS. Fluorenylmethoxycarbonylation of DMA and NA enable highly sensitive quantification by ESI-MS. In this study we measured DMA and NA content in rice xylem sap.
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© 2008 by The Japanese Society of Plant Physiologists
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