Abstract
Iron uptake in roots is predominantly regulated by promotive long-distance signals sent from whole body of a plant. When iron-deficient leaves of Arabidopsis were excised, the expressions of AtIRT1 and AtFRO2 in roots were decreased. However, the expressions of AtbHLH038 and AtbHLH100 were not decreased within 6 hours after the leaf-excision though it is known that their expressions are upregulated by iron deficiency. These results indicate that the former are influenced by the long-distance signals and the latter are influenced by the local signals. Furthermore, to identify novel genes related to the long-distance signals for iron uptake, we comprehensively analyzed RNA abundance in Arabidopsis shoots using the HiCEP (High Cleavage Expression Profile). Some transcripts were significantly accumulated or reduced depending on the iron condition of plants. Similar results were obtained by the real-time PCR analysis.
