Abstract
Vacuole of plant cells is known to be a storage organelle of various metabolites. However, a diversity of metabolites in a vacuole and regulation mechanisms controlling metabolite exchange between vacuole and cytosol have not been elucidated. In this study, we performed a comprehensive annotation of metabolites in the isolated vacuoles and suspension cultured cells of Arabidopsis, to elucidate basic profiles of vacuolar and cytosolic metabolites.
We analyzed extracts from Arabidopsis suspension cultured cells, Deep, and the vacuoles isolated from the cells using liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR-MS).
We detected 495 and 607 metabolites from the isolated vacuoles and the whole cells, respectively. Patterns of molecular size distribution were similar in the vacuoles and the whole cells. A larger number of metabolites were detected in the whole cells than the vacuoles. This work was supported by CREST, JST.
