Abstract
FOX Hunting System is a technique for large-scale gene function analysis. FT-NIR spectroscopy is a method to analyze the metabolite pattern by irradiating the light (4000-10000cm-1) to the sample, and analyze the sample composition based on the spectra obtained from the reflected light. This method is applicable to the un-targeted analysis of limited amount of sample by high-throughput screening. In this research, we performed nondestructive screening by metabolic fingerprinting for FOX lines over-expressing rice full-length cDNA in Arabidopsis thaliana. Two hundred seeds of T2 generation were analyzed by FT-NIR spectroscopy. Subsequently, multivariate analysis was performed to select candidate exhibiting different metabolite profiles. So far, about 3000 lines were analyzed, and 30 lines were selected as candidate. The inserted rice genes were identified, and retransformants of 27 genes were obtained. Currently, reproducibilities of changes in metabolite patterns are assessed by FT-NIR spectroscopy and metabolic profiling will be analyzed by using GC-TOF/MS.
