Development of molecular genetic resources for Marchantia polymorpha: I <crossing, and genetic mapping>

Abstract

Liverworts, including Marchantia polymorpha, are extant species of the first land plant and have simpler body plan than higher plants. Most of the life cycle of M. polymorpha is haploid and dioecious. We focused on the potential of M. polymorpha as a model plant for molecular genetics, and have been developing necessary experimental procedures and resources. We have found far-red-rich growth condition promotes transition to the reproductive phase, which made crosses in laboratory possible. The entire life cycle of M. polymorpha can be completed in three months, and linkage analysis on M. polymorpha requires an F1 population instead of F2, due to its haploidy. Taking advantage of these characteristics, we are constructing a linkage map based on DNA polymorphisms between our two laboratory lines and already mapped >50 markers. A comprehensive linkage map should greatly aid molecular genetics of this unique model plant.