Abstract
Spatially and temporally controlled intercellular attachment via cell wall is indispensable for the organized development. To understand the control mechanism of such attachment, we have established a novel system for mutant production with in vitro culture of leaf disks of haploid Nicotiana plumbaginifolia, and shown that the pectin glucuronyltransferase 1 gene is essential for the formation of the RG-II-B complex, resulting in tight intercellular attachment in meristematic tissues, and is also required for fertilization and reproductive tissue development, including the formation of pollen and pistil transmitting tissue and elongation of the pollen tube through the pistil. In another system, morphological and gene expression analyses during tissue-reunion were performed using the Arabidopsis cut flowering stem. Cell division was initially observed in 3 days and tissue-reunion almost completed in14 days. The microarray analysis showed a number of genes involved in cell division, cell wall-modification, hormone-synthesis and signal transduction were up-regulated.
