Abstract
Lipid peroxidation plays a role in the pathogenesis of many types of tissue injuries and especially in the tissue damage induced by several toxic substances. The important lipid peroxidation products that have been extensively studied include short-chain aldehydes, such as ketoaldehydes, 2-alkenals, 4-oxo-2-alkenals, and 4-hydroxy-2-alkenals. Protein modification by these aldehydes generates protein carbonyls, which represent a putative marker of oxidatively modified proteins. Taking advantage of the fact that protein-bound aldehydes are excellent immunogens that are capable of stimulating adaptive immune response, a number of monoclonal antibodies have been developed. In this presentation, I will summarize our chemical and immunochemical approaches that have been explored for protein-bound lipid peroxidation products as biomarkers. I will also provide an update of our latest approach to establish the involvement of aldehydes in the etiology of human diseases.
