Abstract
To perform comparative sequence and transcriptome analyses between Brassica rapa and Arabidopsis thaliana, we prepared a B. rapa cDNA microarray using ca. 2 K cDNA clones selected from 2,166 non-redundant sequences of cDNA library of Chinese cabbage. The gene expression during infection with fungal pathogen Colletotrichum higginsianum and treatments with signaling molecules was analyzed using 2 K B. rapa and 1.2 K Arabidopsis cDNA microarrays. In B. rapa and A. thaliana, the results showed a large correlation coefficient between compatible pathogen C. higginsianum-infection and the treatment with salicylic acid but small correlation between the pathogen and methyl jasmonate (MeJA)-treatment. From the results, it is clarified that the disease was perfectly controlled by pretreatment of Chinese cabbage with BTH but not with MeJA, which inversely accelerated lesion development. These expressed sequence tag (EST) and microarray data should provide a valuable resource for functional genomics on the crops.
