Host: The Japanese Society of Plant Physiologists
Pages 0003
During leaf development, growth regulatory factors should localize correctly in adaxial- or abaxial-side of the primodia. However, the mechanisms of the adaxial-abaxial boundary formation are still unknown. To reveal the mechanisms, we focused on the expression domains of PHB, FIL and miR165/166 in Arabidopsis leaf primodia. The analysis of promoter-reporter fusion genes indicated that the expression domains of PHB and FIL were partially overlapped. In contrast, the overexpression of GFP, whose expression was suppressed by miR165/166, showed the adaxial-abaxial boundary. Then, using laser-microdissection technique, total RNA was prepared from the adaxial- or abaxial-side of the primodia, respectively, and the localizations of pre-miR165/166 were checked by semi-qRT-PCR analysis. Among 9 genes, 6 transcripts were found only in abaxial-side. Thus, we concluded that the adaxial-abaxial boundary is determined by suppression of PHB expression via abaxial-specific function of miR165/166. We will discuss about the suppression mechanisms in the meeting.
