Characterization of enf1 Mutant, which is Defective in Shoot Apical Meristem and Adaxial-abaxial Axis, by Transcriptome and Metabolome Analyses

Abstract

Development of shoot apical meristem (SAM) and the adaxial-abaxial (ad-ab) axis of leaf primordial is important for shoot development. It is known that SAM and ad-ab axis mutually control their development. We isolated enf1 mutant, which is defective in both SAM and the ad-ab axis. The expression patterns of the key transcription factors, FIL and WUS were altered in enf1 mutant, suggesting that ENF1 regulate SAM and ad-ab axis via transcriptional regulation of the transcription factors. Transcriptome analysis also revealed that ENF1 regulated several transcription factors. ENF1 gene encoded an enzyme in the primary metabolism, and metabolome analysis showed a drastic change of metabolites in SAM of the mutant. It is suggested that the metabolic condition of cells is responsible to gene expression profile. We will discuss the relationship among metabolite, transcription, and morphogenesis.