Annotations of metabolites in the isolated vacuoles obtained from suspension cultured cells of Arabisopsis

Abstract

Vacuole of plant cells is a storage organelle of various metabolites. However, an actual diversity of metabolites in a vacuole has not been clarified. In this study, we performed a comprehensive annotation of metabolites in the isolated vacuoles from suspension cultured cell of Arabidopsis (DEEP stain) using LC-FTICR-MS, to elucidate basic metabolite profile of vacuole. A metabolite profile of the whole cell fraction was also analyzed. From the isolated vacuoles and the whole cells, 736 and 876 metabolites were detected in positive mode, respectively, and 713 and 989 metabolites were detected in negative mode, respectively. The whole cell extract contained a larger number of metabolites in a m/z range smaller than 500 Da. Interestingly, several metabolites in nucleic acid metabolism and amino acid metabolism were detected exclusively in the isolated vacuole or the whole cell. This work was partly supported by CREST, JST.