Abstract
It has been known that metabolite concentrations are different among not only each cell in organism but also organelles in a cell. However, because metabolite amounts in a single cell and organelle are too small to detect, direct determination of actual conditions of metabolites in a cell is quite difficult. To overcome this problem, we have utilized a giant internodal cell of Chara corallina for a comprehensive metabolomic analysis in a single cell. For metabolomic analysis, we have used CE-MS (capillary electrophoresis / mass spectrometer) which is useful for the separation and detection of ionic compounds. In this study, we identified 55 metabolites in a single cell. Additionally, we conducted metabolomic analysis of a single vacuole and cytoplasm easily isolated from a Chara internodal cell. Metabolomic fluctuations in a cell and organelles under different light conditions were examined.
