Metabolome And Transcriptome Analyses Of Molybdenum Deficiency Responses In Arabidopsis thaliana

Abstract

Molybdenum (Mo) is a microelement essential for plant growth. Nitrate reductase, sulfite oxidase, abscisic aldehyde oxydase and xanthine dehydrogenase are Mo-cofactor (MoCo) containing enzymes in plants. Molybdate transporter MOT1 plays an important role in efficient Mo uptake in Arabidopsis thaliana. We performed microarray experiment using A. thaliana wild-type (Col-0) and mot1-1, a T-DNA inserted mutant with reduced Mo accumulation. Plants were grown on solid media with sufficient or deficient Mo for 18 days. We analyzed the metabolic profiles of the plants by GC-TOF/MS and CE-TOF/MS. A number of metabolites and mRNA accumulations were altered significantly by Mo deficiency in mot1-1.