Analysis of gene regulation via Arabidopsis membrane-bound NAC transcription factor(HLN) under high-light stress conditions

Abstract

We isolated a high-light inducible NAC transcription factor (HLN) by suppression-subtractive hybridization from Arabidopsis. The HLN protein had a highly conserved NAC DNA binding domain in the N-terminal region and a transmembrane (TM) motif in the C-terminal region. The GFP fused full-length HLN (GFP-HLN) protein was localized in cytosol and nucleus, while the truncated form of HLN without TM motif (HLNΔTM) was only in nucleus, suggesting that HLN is released from membrane and translocated to nucleus under high-light stress conditions.
The transcriptome analysis using the HLN-overexpressing (Ox-HLN) Arabidopsis plants showed that the transcript levels of 166 genes are induced and those of 470 genes are suppressed compared with wild-type plants under high-light stress conditions.