Abstract
The DREB1A gene of Arabidopsis (AtDREB1A) is an important transcriptional factor that controls many abiotic stress-related genes and confers abiotic stress tolerance on several plant species. From our previously study, AtDREB1A gene conferred abiotic stress tolerance to potato. This result suggests potato also has the system for abiotic stress tolerance controlled by DREB1A. However, the mechanism underlying this system is not understood.
In this study, to elucidate the mechanism of abiotic stress tolerance controlled by DREB1A in potato, we analyzed gene expression profile of non-transgenic potato under abiotic stress condition and that of CaMV35S::AtDREB1A transgenic potato using an Oligo-based DNA microarray. Microarray analysis showed that 98 genes were up-regulated and 19 genes were down-regulated. In addition, to show the correlation between gene expression profile and abiotic stress tolerance in more detail, RT-PCR analyses were also performed.
