Abstract
ANGUSTIFOLIA (AN; 636 aa), a plant homolog of CtBP/BARS, controls the polar elongation of leaf cell and trichome-branching pattern in Arabidopsis thaliana. AN has a plant-specific long C-terminal region of about 200 aa. Gene encoding AN without this long C-terminus was not found in A. thaliana genome. To decide functions of AN homologs in moss, we determined the full-length cDNA sequences of four Physcomitrella patens (Pp)AN genes (1-1, 1-2, 2-1, 2-2). Homologs of PpAN1-1 and 1-2 have the long C-terminus, while PpAN2-1 and 2-2 do not contain. PpANs show high similarity to AN: 44.1%, 45.5%, 35.3% and 35.0% for PpAN1-1, 1-2, 2-1 and 2-2, respectively.Single KO lines with insertion of drug-resistant gene were produced for each PpAN genes by a gene-targeting technique. At present, we can not observe any changes in P. patens morphology.Production of double KO lines with loss of a pair of genes is in progress
