Abstract
Plant hormones play an important role as signaling molecules in the regulation of almost all phases of plant development, from seed germination to senescence. They regulate expression of genes via each signal transduction systems, and in most cases they mutually regulate the signaling and metabolic systems between the hormones. To understand the fine regulatory systems of hormone levels, we have established a method for high-sensitive and high-throughput analysis of major plant hormones, namely, cytokinins (23 species), auxins (7 species), abscisic acid, and gibberellins (12 species). Our method needs 10 to 100 mg fresh weight of plant tissues for determination and enables us to analyze 180 samples at a time. We are now trying to identify key genes regulating hormone levels in rice using QTL analysis. We have measured hormone contents of Sasanishiki X Habataki BILs. The data will be presented.
