Abstract
Among PM Qa-SNARE molecules expressed during root development in Arabidopsis, SYP123 shows root-hair cell specific expression and polarized accumulation at root hair tip region, whereas SYP132 expresses constitutively in all root cell types so far examined. Here we performed the live imaging analysis coupled with the treatment of various inhibitors of membrane trafficking, and examined whether some differences in intracellular dynamics are observed between these PM Qa-SNAREs. We further elucidated the interaction specificity among PM-SNAREs to form complexes using the in vitro complex formation assay and the Split Luciferase complementation assay. From these analyzes, we found the candidate R-SNARE molecules which might be involved in a membrane traffic pathway to tip-region of root hair. In addition, we also present the tissue-selective expression pattern or subcellular dynamics of mRFP-R-SNARE was similar to that of Qa-SNARE. These results indicated the existence of vesicular trafficking mechanism with complicated balance of exocytosis and endocytosis.
