Biochemical analysis of alleles of d1, which are a deficient mutant of a heterotrimeric G protein α subunit gene in rice

Abstract

We studied the transcripts and translation products of the rice Gα in ten alleles of d1, which was a deficient mutant of the α subunit (Gα) gene of heterotrimeric G protein in rice. The transcripts of the Gα gene were analyzed by RT-PCR in all d1 alleles. The Gα proteins were not detected in the plasma membrane fractions by western blot using anti Gα antibody, in the nine d1 alleles except d1-4, in which one amino acid exchange in GTP-binding box (A) was happened. The Gα protein was barely present in d1-4. Gα proteins were not present in d1-8, which one amino acid deletion in the effector binding region 2 was happened. From the results, it is evoked that a proper conformation of Gα is necessary for accumulation of Gα in the plasma membrane.