Abstract
Cyclobutane pyrimidine dimer (CPD) photolyase is a crucial factor for determining UVB sensitivity in plants. CPD photolyases are categorized into two groups, class I in microorganisms and class II in higher organisms, on the basis of their deduced amino acid sequences. Plant CPD photolyase is classified as class II group. However, biochemical characteristics of class II CPD photolyase of plant are unclear. To analyze the characteristics of CPD photolyase, we purified the CPD photolyase from rice leaves, and found the native rice CPD photolyase is phosphorylated. There is no report that CPD photolyase is phosphorylated in organism other than rice. Then, we established the new method for purification of CPD photolyase from rice leaves using a UV irradiated DNA-conjugates magnetic beads, and analyzed the phosphorylation on CPD photlyase in several plants. In this study, we discuss about the significance of phosphorylation on CPD photolyase in plants.
