Abstract
To development gene resources related to UVB tolerance of rice, UVB-resistant rice cultivar Sasanishiki was irradiated with carbon ion beam (320 MeV:12C6+, 80Gy). We isolated three UVB hyper-resistant and two hyper-sensitive mutants, compared with UVB-resistant Sasanishiki. In this study, we focused one of UVB hyper-resistant mutant, UVTSa-319 (UV-tolerant Sasanishiki-319) and analyzed the characteristics of the mutant. Consequently, there were no differences in the amount of UV-absorbing compounds, the activities of cyclobutane pyrimidine dimers and (6-4) photoproducts photorepair and dark repair activity between wild type Sasanishiki and UVTSa-319. Next, we performed Array Comparative Genomic Hybridization (CGH) to estimate mutated region. As a result, we found that about 40 kbp on chromosome 7 containing 2 genes which has unknown function was deleted in UVTSa-319. We discuss about possibility of new UV tolerance gene resource.
