Abstract
Obligate biotroph powdery mildew is one of the most economically important fungal pathogens. Powdery mildew infection is limited to the plant tissue surface, and its infection is highly race-specific. Compatible powdery mildew overcomes plant defense response at various levels to establish colonization successfully. Forward genetics has been identified a number of factors that are involved in defense response against powdery mildew infection. However, the functions of many of those factors still remain to be elucidated. To identify Arabidopsis thaliana novel factors involved in the resistance against compatible powdery mildew Golovinomyces orontii, we performed 2-DE/LC-MS/MS analyses on powdery mildew-infected and non-infected leaves.
With this new approach, we have identified a number of proteins, which are likely involved in plant resistance. We will report here the results of 2-DE/LC-MS/MS analyses. In addition, investigation on the spatiotemporal expression pattern of those identified factors and genetic analyses are underway.
