Abstract
Jasmonic acid (JA) is a plant hormone related to responses for biotic and abiotic stresses such as wounding and pathogenesis. JAZ proteins repress JA response by binding to MYC2 transcription factor, which is positive regulator of JA signaling. In the presence of JA, binding of JA-derivative to COI1 leads to degradation of JAZ proteins followed by activation of downstream JA signaling. Although involvement of other transcription factors in JA signaling has been speculated, such transcription factors have not yet been identified.
CRES-T is gene silencing technology, which efficiently suppresses functions of not only an endogenous but also functionally-redundant transcription factors. In order to identify transcription factors that are involved in JA signaling pathway, we screened CRES-T transgenic plants with impaired JA-responsiveness, and isolated several JA-insensitive CRES-T transgenic plants. Several of them were male-sterility.
