Characterization of CEBiP2 possessed a structure similarity with chitin elicitor receptor in rice cells.

Abstract

CEBiP as a receptor plays an important role for chitin specific elicitor signaling in rice cells. However, the result from microarray analysis of CEBiP-RNAi suggests that the possibility of other molecule also assist the same function as like CEBiP. Meanwhile, we found a minor protein, named CEBiP2, was co-purified with CEBiP by (GlcNAc)₈-Sepharose. CEBiP2 is a membrane glycoprotein consisted with two LysM motif in extracellular and three amino residues in intercellular domain, which showed the high structure similarity with CEBiP. To survey the function of CEBiP2, we prepared the CEBiP2 specific over-expression and knock down transformated rice cell. The result shows that CEBiP2 possesses the chitin elicitor binding activity. Moreover, the CEBiP2 specific knock-down cell lines were reduced the generation of chitin elicitor specific induced ROS. These results indicate that CEBiP2 may also play a same function as same as CEBiP.