Abstract
Acacia mangium is a fast-growing tree and widely planted in Southeast Asia. The tree improvement program by elite tree selection is underway, but the molecular breeding based on genetic information is not progressed. In this research, EST analysis in A. mangium was carried out to get essential knowledge base for molecular breeding of A. mangium.
Total RNA was extracted from developing xylem and shoot in A. mangium. cDNAs were generated by reverse-transcription and normalized. Normalized cDNAs were ligated into vectors, and the resulting plasmids were electroporated into E. coli cells. Independent 10,752 colonies of E. coli were randomly picked, and the inserts were amplified by PCR. The amplified inserts were sequenced by Sanger method. The derived sequences were submitted to BLASTX analysis, and 7,710 sequences shared similarity with the sequences deposited in SwissProt database. Over half (60%) of the sequences was full-length cDNAs. We are currently annotating the EST sequences.
