Abstract
A miniature cultivar Micro-Tom has attracted much attention as a model of tomato (Solanum lycopersicum). We have constructed five cDNA libraries and four full-length cDNA libraries from Micro-Tom, and generated ESTs and full-length cDNAs. By assembling Micro-Tom ESTs with publicly available tomato ESTs, a non-redundant sequence set (unigenes) is obtained. At present, KTU3 (Kazusa Tomato Unigene ver. 3) have been constructed and annotated with BLAST-based similarity. A database MiBASE (http://www.kazusa.or.jp/jsol/microtom/) have been maintaining to provide information of Micro-Tom ESTs and KTUs, including DNA markers, BLAST annotations, Gene Ontology terms, metabolic pathways and gene expressions obtained from Micro-Tom cDNA microarrays. To elucidate intron-exon structures of the tomato genome, we also compare the full-length cDNAs with genomic sequences from the international tomato genome sequencing project. All information of the genome annotations is available from a database KaFTom (http://www.pgb.kazusa.or.jp/kaftom/).
