Analysis of lipid recycling using C-13

Abstract

Recycling of acyl group of glycerolipids remained obscure, because direct analysis of recycling of membrane lipids has been difficult. To analyze acyl recycling in glycerolipids, a computational methodology for the analysis of mass spectra of C-13 labeled molecules was developed. In the present study, some representative data will be presented to show the existence of acyl exchange without changing fatty acid composition of lipids. Based on the results, I will propose a new hypothesis of lipid flow within the membrane.