Abstract
Cyanobacteria are representative phototrophic organisms, so it should regulate metabolism flexibly. Although many studies for regulation of metabolism, such as photosynthesis, use these organisms, few studies have reported the amount of metabolites and its variation in these organisms. To investigate how cyanobacteria regulate metabolic reprogramming in poor nutrient conditions, we quantified amino acids pool that are center of carbon, nitrogen and sulfur metabolism. We collected cyanobacterial cells by centrifugation or filtration and froze in liquid nitrogen. The cell pellet was extracted with methanol. The extracts were dried up and dissolved in water. The dissolved extracts were applied to solid phase extraction, derivatized, and analyzed by gas chromatography mass spectrometry (GC-MS). We will report responses in amino acids pool to nitrogen or sulfur starvation.
