Abstract
It is empirically known that cyanobacteria easily give rise to spontaneous mutations on their genome sequences. These differences of genotype and phenotype between the laboratory strain and the strain whose genome had been sequenced sometimes cause severe problems on post genome analyses. To solve this problem, it is necessary to resequence the genome of laboratory strains. Here, we carried out resequence analysis of the laboratory strain of a cyanobacterium, Synechococcus elongatus PCC 7942 using next generation sequencer, Genome Analyzer II. Mapping analysis of 700 Mb data of short-read sequences to the reference genome enables us to identify 7 SNPs and a large deletion of continuous 48.7 kb region in the laboratory strain. These results suggest that the importance of resequence analysis on the bacterial genome research.
