Analysis of target proteins ubiquitinated by ATL31 in Arabidopsis thaliana

Abstract

Carbon and nitrogen availability is one of the most important factors to regulate plant development. To utilize limited resource of carbon and nitrogen efficiently, plants enable to sense and respond to balance of carbon (C) and nitrogen (N) metabolites, called C/N response.
We previously showed a novel ubiquitin ligase ATL31 has essential role to regulate C/N response in Arabidopsis thaliana. In this study, we tried to isolate target protein ubiquitinated by ATL31. As a result of immunoprecipitation and MS analysis used epitope-tagged ATL31, we found several candidates of target protein for the ubiquitination. Details of the ubiquitinated proteins and biochemical function of ATL31 will be discussed.