Abstract
It is well known that pavemant cells show their jigsaw puzzle-like shapes in leafe epidermis of dicotyledonous plants. However, intracellular dynamics during the cell morphogenesis are largely unknown. We found the interesting localization of mRFP-tagged sialyl transferases (ST-mRFP), which label the cytosolic side of trans-Golgi membrane. ST-mRFP fluorescence was observed in the pavement cell border, whereas it was also in trans-Golgi bodies as previously reported. In guard cells, ST-mRFP strongly labeled the both ends of the guard cells. We confirmed that ST-mRFP localized in apoplast by observation of the plasmolyzed cells and dual observation with plasma membranes by vital FM4-64 staining. The observation in various leaf growth stages showed that the apoplastic localization become pronounced during cell maturation. Also, our image analysis techniques quantitatively revealed that the fluorescence intensity has strong correlation with the curvature of the cell border, suggesting that ST-mRFP tended to localize at the curved region.
