Abstract
Recent progress on microscopy techniques allows us to provide tremendous amounts of microscopy data that can easily surpass the volume of omics data. We therefore believe that there will be a global trend toward the development of microscopy image databases and data mining technologies. We have obtained serial optical sections of various fluorescent-labeled structures in Arabidopsis guard cells, and made the image sets public as LIPS (Live Images of Plant Stomata) database. In this study, we performed localization analysis using statistical approaches. We visualized and analyzed the intracellular distribution of the structures by the averaged intensity projection (AIP) image obtained from 100-120 images. These colocalizations were quantitatively evaluated by the intensity correlation coefficient among the AIP images. From a series of the studies, it has been suggested that microtubules and mitochondria mainly localize in the proximal side, whereas actin microfilaments and endosomes localize in the distal side. Also, actin microfilaments and endoplasmic reticulum were observed in the connection of the guard cells.
